Bioimaging is a noninvasive process of visualizing biological activity in real time.
It does not inhibit the various life processes and helps to report the
4D dynamics of subcellular structures without inferencing physically.
We are dedicated to developing experimental solutions that can suit various types of biological systems relevant to basic and applied research in the agricultural, pharmaceutical, or biotechnological sectors.
With our multi-use products, fabricated with biopolymers from renewable, plant-based resources, we aim to make an important contribution to the reduction of non-biodegradable plastics spend in laboratory use and, hence, to environmental sustainability.
Research & Development
We work in close collaboration with international academic and industrial research groups to developed novel experimentation tools for live-cell imaging and live- cell physiology.
We currently focus on implementing solutions to measure the influences of light, diffusion, secretion and volatile organic compounds on cell physiology.
Novel ideas and challenges are always welcome. A development pipeline for customised products, designed according to specific client requirements, is being set up.
Applications I ib01
The preparation of microcolonies inside ib01 chambers provides superior imaging of fungal interactions on the cellular level. (A) Petri dish confrontation culture between the plant pathogen Fusarium oxysporum (purple) and the mycoparasite Trichoderma asperellum (green). (B) Close up of the interaction zone. (C) Microcolony confrontation culture prepared inside a inncelly ib01 chamber provides superior live-cell imaging conditions. Scale bars, 1 cm. (D) CRIB reporters (arrowheads) visualise mycoparasitic hyphae (green) attacking the prey fungus (yellow). (E) Time projection of the dynamic displacement of GTPase activity in the growing tip apex (arrowhead) and the Spitzenkörper (Spk, arrow). Scale bars, 10 μm.
Applications I il30
Application examples of wavelength-dependent cellular responses using inncelly il30 chambers. Only blue light between 455-465 nm properly induces (A) the formation of green spores in T. atroviride and (B) nuclear import of the GFP-labelled blue light receptor VVD in N. crassa (Chen et al., 2010. PNAS 107(38)). (C) Image quantification reveals that GFP-VVD shuttling occurs almost twice as efficiently in response to 455-465 nm compared to 425-435 nm, and that the inducing effect is additive under white light.
Applications I ib02
Wavelength dependent colony development in inncelly ib02 chambers. Blue light between 455-465 nm (460/10 nm) not only induces the formation of green-yellow spores in T. atroviride (Ta), but also prevents the mycoparasite from invading the mycelium of its putative prey F. oxysporum (Fo). When forced to grow under 605-615 nm illumination (610/10 nm), T. aroviride cannot properly induce conidiogenesis. but is able to overgrow F. oxysporum.